Życiorys naukowy ALEKSANDER L. SIEROŃ

Studia
Wydział Biologii i Ochrony Środowiska, Uniwersytet Śląski, Katowice (mgr biologii)
Tytuł
Profesor, doktor habilitowany nauk medycznych
Doktorat -1990
"Dynamika zmian składu białkowego frakcji submikrosomalnej w nerwach kulszowych szczurów, w zależności od czasu po przecięciu" 1989, Śląska Akademia Medyczna, Katowice.
Habilitacja- 2002
"Regulacja aktywności C-proteinazy i N-proteinazy prokolagenowej podczas angiogenezy" 2001, Śląska Akademia Medyczna, Katowice
Staże naukowe:
po doktoracie - Thomas Jefferson University, Filadelfia, USA, 1991 do 1994
Przebieg pracy naukowej

Publikowane prace:

1.              Sieron A., Lewin-Kowalik J. (1988) [Nerve growth factor, its chemical structure and biological function.] Postępy Biochemii, 34:173-82.

2.              Lewin-Kowalik J., Sieron A.L., Krause M., Kwiek S. (1990) Predegenerated peripheral nerve grafts facilitate neurite outgrowth from the hippocampus. Brain Research Bulletin, 25:669-73. (IF=1,977)

3.              Sieron A., Lewin-Kowalik J., Krause M., Fertala A. (1991) Time-dependent dynamic changes of protein content and composition in the submicrosomal fraction from the rat sciatic nerve following transection. Annales Academie Silesiensis, 23:81-9.

4.              Lewin-Kowalik J., Sieron A.L., Krause M., Barski J.J., Gorka D. (1992) Time-dependent regenerative influence of predegenerated nerve grafts on hippocampus. Brain Research Bulletin, 29:831-5. (IF=1,977)

5.              Lewin-Kowalik J., Sieron A.L., Barski J.J. (1992) [Recent views on the role of nerve growth factor and other molecules with well documented neuronotrophic action] Postępy Higieny i Medycyny Doswiadczalnej, 46:497-519.

6.              Barski J.J., Lewin-Kowalik J., Sieron A.L. (1992) [Current views on the structure and function of the hippocampus]. Neurologia i Neurochirurgia Polska, 26:224-31.

7.              Lewin-Kowalik J., Sieron A.L., Krause M., Barski J.J., Gorka D. (1992) The Influence of peripheral nerve graft's predegeneration stage on the regrowth of hippocampal injured neurite and concomitant changes in submicrosomal fraction proteins of grafts. Acta Physiologica Hungarica, 79:219-31. (IF=0,208)

8.              Fertala A., Sieron A.L., Ala-Kokko L., Prockop D.J. (1993) Expression of normal and mutated type II procollagen genes in transfected human tumor cells (HT-1080). Matrix. Collagen Related Research, 13:15. (IF=3,214)

9.              Sieron A.L., Fertala A., Ala-Kokko L., Prockop D.J. (1993) Deletion of a large domain in recombinant human procollagen II does not alter the thermal stability of the triple helix. J. Biol. Chem., 268:21232-7. (IF=7,666)

10.          Fertala A., Sieron A.L., Ganguly A., Li S-W., Ala-Kokko L., Anumula K.R., Prockop D.J. (1994) Synthesis of recombinant human procollagen II in a stably transfected tumor cell line (HT-1080). Biochem. J., 298(Pt 1):31-7. (IF=4,349)

11.          Fertala A., Sieron A.L., Hojima Y., Ganguly A., Prockop D.J. (1994) Self assembly into fibrils of collagen II by enzymic cleavage of recombinant procollagen II. Lag period, critical concentration, and morphology of fibrils differ from collagen I. J. Biol. Chem., 269:11584-9. (IF=7,666)

12.          Ganguly A., Smelt S., Mewar R., Fertala A., Sieron A.L., Overhauser J., Prockop D.J. (1994) Targeted insertions of two exogenous collagen genes into both alleles of their endogenous loci in cultured human cells: the insertions are directed by relatively short fragments containing the promoters and the 5' ends of the genes. Proc. Natl. Acad. Sci. U S A, 91:7365-9. (IF=10,260)

13.          Jagodzinski P.P., Wustner J., Kmieciak D., Wasik T.J., Fertala A., Sieron A.L., Takahashi M., Tsuji T., Mimura T., Fung M.S., Gorny M.K., Kloczewiak M., Kaneko Y., Kozbor D. (1996) Role of the V2, V3, and CD4-binding domains of GP120 in curdlan sulfate neutralization sensitivity of HIV-1 during infection of T lymphocytes. Virology, 226:217-27. (IF=3,548)

14.          Arnold, W.V., Sieron, A.L., Prockop, D.J. (1996) Creation of a system for the assembly and expression of DNA constructs encoding novel recombinant human procollagen II. Annals of the New York Academy of Sciences, 785:204-5.

15.          Fertala A., Holmes D.F., Kadler K.E., Sieron A.L., Prockop D.J. (1996) Assembly in vitro of thin and thick fibrils of collagen II from recombinant procollagen II. The monomers in the tips of thick fibrils have opposite orientation from monomers in the growing tips of collagen I fibrils. J. Biol. Chem., 271:14864-9. (IF=7,666)

16.          Li S.W., Sieron A.L., Fertala A., Hojima Y., Arnold W.V., Prockop D.J. (1996) The C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1. Proc. Natl. Acad. Sci. U S A, 93:5127-30. (IF=10,260)

17.          Colige A., Li S-W., Sieron A.L., Nusgens B.V, Prockop D.J., Lapiere C.M. (1997) cDNA cloning and expression of bovine procollagen I N-proteinase. A new member of the superfamily of zinc-metalloproteinases with binding sites for cells and other matrix components. Proc. Natl. Acad. Sci. U S A, 94:2374-9. (IF=10,260)

18.          Arnold W.V., Sieron A.L., Fertala A., Bachinger H-P., Mechling D., Prockop D.J. (1997) A cDNA cassette system for the synthesis of recombinant procollagens. Variants of procollagen II lacking a D-period are secreted as triple-helical monomers. Matrix Biology, 16:105-16. (IF=3,214)

19.          Zafarullah K., Brown E.M., Kuivaniemi H., Tromp G., Sieron A.L., Fertala A., Prockop D.J. (1997) Synthesis and conformational properties of a recombinant C-propeptide of human type III procollagen. Matrix Biology, 16:201-9. (IF=3,214)

20.          Zafarullah K., Sieron A.L., Brown E.M., Kuivaniemi H., Tromp G., Fertala A., Prockop D.J. (1997) A recombinant homotrimer of type I procollagen that lacks the central two D-periods - the thermal stability of the triple helix decreased by 2 to 4 degrees - C. Matrix Biology, 16:245-53. (IF=3,214)

21.          Krynska B., Lewin-Kowalik J., Sieron A.L. (1998) [Disturbance of the normal cell cycle by T antigens of SV40 and JC viruses causes some tumors]. Postępy Hig. Med. Dośw. 52:237-57.

22.          Prockop D.J., Sieron A.L., Li S.-W. (1998) Procollagen N-proteinase and procollagen C-proteinase - two unusual metalloproteinases that are essential for procollagen processing probably have important roles in development and cell signaling Matrix Biology. 16:399-408. (IF=3,214)

23.          Arnold W.V., Fertala A., Sieron A.L., Bachinger H-P., Mechling D., Prockop D.J. (1998) Recombinant procollagen II. Deletion of D-period segments identifies sequences that are required for helix stabilization and generates a temperature-sensitive N-proteinase cleavage site. J. Biol. Chem. 273:31822-8. (IF=7,666)

24.          Colige A., Sieron A.L., Li S.-W., Schwarze U., Petty E., Wertelecki W., Wilcox W., Krakow D., Cohn D.H., Reardon W., Byers P.H., Lapiere C.M., Prockop D.J., Nusgens B.V. (1999) Human Ehlers-Danlos Syndrome type VII C and bovine dermatosparaxis are caused by mutations in the procollagen I N-proteinase gene. Am. J. Hum. Genet. 65:308-17. (IF=10,426).

25.          Sieron A.L., Tretiakova A., Jameson B., Segall M.L, Lund-Katz S., Khan M.T., Li S.-W., Stöcker W. (2000) Structure and function of procollagen C-proteinase (mTolloid) domains defined by protease digestion, circular dichroism, binding to procollagen type I, and computer modeling. Biochemistry, 39:3231-9. (IF=4,493).

26.          Kramerova I.A., Kawaguchi N., Nelson R.E., Fessler L.I., Kramerov A.A., Kusche-Gullberg M., Kramer J.M., Sieron A.L., Prockop D.J., Fessler J.H. (2000) Papilin in development: an essential pericellular protein with a homology to the ADAM-TS metalloproteinases. Development, 127:5475-85. (IF=10,088).

27.          Fertala A., Sieroń A.L., Adachi E., Jimenez S.A. (2001) Collagen II containing a Cys substitution for Arg-alpha1-519: abnormal interactions of the mutated molecules with collagen IX. Biochemistry, 40:14422-8. (IF=4,114).

28.          Kiszka I., Kmieciak D., Gzyl J., Naito T., Bolesta E., Sieron A., Singh S.P., Srinivasan A., Trinchieri G., Kaneko Y., Kozbor D. (2002) Effect of the V3 loop deletion of envelope glycoprotein on cellular responses and protection against challenge with recombinant vaccinia virus expressing gp160 of primary human immunodeficiency virus type 1 isolates. J. Virol. 76:4222-32. (IF=5,241).

29.          Sieron A.L., Louneva N., Fertala A. (2002) Bone morphogenetic protein 2 interacting with fibrillar procollagen. Cytokine, 18:214-21. (IF=2,374).

30.          Szilman E., Szilman P., Solarz K., Brewczyński P., Sieroń A.L. (2004) Sensitization to the storage mite Tyrophagus putrescentiae in urban population of Upper Silesia (Poland).  Wiad Parazytol. 50:471-6.

31.          Sieroń A.L., Stańczak P. (2006) ASD lessons on genetic background from transgenic mice with inactive gene encoding metalloprotease, Tolloid-like 1 (TLL1). Med Sci Monit. 12:RA17-22. (IF=1,595).

32.          Tarnowski M., Sieron A.L. (2006) Adult stem cells and their ability to differentiate. Med Sci Monit. 12:RA154-63. (IF=1,595).

33.          Tarnowski M., Koryciak-Komarska H., Czekaj P., Sebesta R., Czekaj T.M., Urbanek K., Likus W., Malinowska-Kolodziej I., Plewka D., Nowaczyk-Dura G., Wiaderkiewicz R., Sieron A.L. (2007) The comparison of multipotential for differentiation of progenitor mesenchymal-like stem cells obtained from livers of young and old rats. Folia Histochem Cytobiol. 45:245-54. (IF=0,886).

34.          Grzybowska-Chlebowczyk U, Woś H, Sieroń AL, Kajor M, Wiecek S, Koryciak-Komarska H, Auguściak-Duma A. (2008) [Parenteral symptoms and intestinal complications in children with inflammatory bowel diseases in relation to card15 mutation] Med Wieku Rozwoj. 12:754-60.

35.          Auguściak-Duma A., Sieroń A.L. (2008) [Molecular characteristics of leiomyoma uteri based on selected compounds of the extracellular matrix] Postepy Hig Med Dosw. 62:148-65.

36.          Lesiak M., Augusciak-Duma A., Szydlo A., Pruszczynska K., Sieron A.L. (2008) Specific inhibition of procollagen C-endopeptidase activity by synthetic peptide with conservative sequence found in chordin. Acta Biochim Pol. 55:297-305. (IF=1,448).

37.          Witecka J., Auguściak-Duma A.M., Kruczek A., Szydło A., Lesiak M., Krzak M., Pietrzyk J.J., Männikkö M., Sieroń A.L. (2008) Two novel COL1A1 mutations in patients with osteogenesis imperfecta (OI) affect the stability of the collagen type I triple-helix. J Appl Genet. 49:283-95. (IF=1,351).

38.          Lisik M.Z., Sieroń A.L. (2008) [X-linked mental retardation--treatment scheme] Wiad Lek. 61:146-53.

39.          Tarnowski M., Sieroń A.L. (2008) [Osteogenesis imperfecta--etiology, characteristics, current and future treatment] Wiad Lek. 61:166-72.

40.          Lisik M.Z., Sieron A.L. X-linked mental retardation. (2008) Med Sci Monit. 14:RA221-9. (IF=1,514).

41.          Lisik M, Sieroń AL. (2008) [ARX - one gene - many phenotypes] Neurol Neurochir Pol. 42:338-44.

42.          Auguściak-Duma A., Kajor M., Piwowarczyk M., Sikora J., Sieron A.L. (2009) [Changes of mRNAs encoding alternatively spliced variants of procollagen C-endopeptidase in leiomyomas uteri depending on the phase of menstrual cycle and in postmenopausal women]  Ginekol Pol. 80:25-32.

43.          Stańczak P, Witecka J, Szydło A, Gutmajster E, Lisik M, Auguściak-Duma A, Tarnowski M, Czekaj T, Czekaj H, Sieroń AL. (2009) Mutations in mammalian tolloid-like 1 gene detected in adult patients with ASD. Eur J Hum Genet. 17:344-51. (IF=3,925).

44.          Lesiak M., Auguściak-Duma A., Szydło A., Sieroń A.L. (2009) Blocking angiogenesis with peptides that inhibit the activity of procollagen C-endopeptidase. Pharmacol Rep. 61:468-476. (IF=2,167).   


Wybrane doniesienia na konferencjach naukowych

  1. 1.            Koryciak-Komarska H., Czekaj P., Sebesta R., Zborek A., Wiaderkiewicz R., Sieroń A.L. (2003) Age dependent changes in rat hepatic primary stem cells kontent. Acta Angiologia, 9, Supl. Abstr.:31. (The First Annual Meeting of the Stem Cell Therapeutic Excellence Centra (STEC) Krakow 2003).

    2.            Sieron, A.L., Tarnowski, M., Koryciak Komarska, H., Czekaj, P., Wiaderkiewicz, R. (2004) Mesenchymal stem cells in tissues of adults for gene, cell therapy, and tissue engineering. Proceedings of stem cell research. 1, Suppl.:29. The Second Annual Meeting of the European Stem Cell Therapeutic Excelence Centre (STEC), Cracow June 7-8, 2004.

    3.            Koryciak-Komarska, H., Czekaj, P., Tarnowski, M., Sebesta, R., Wiaderkiewicz, R., Sieron, A.L. (2004) Differentiation of mesenchymal stem cells from liver into osteoblasts, adipocytes, and neuron-like progenitors. Proceedings of stem cell research. 1, Suppl.:45. The Second Annual Meeting of the European Stem Cell Therapeutic Excelence Centre (STEC), Cracow June 7-8, 2004. 

    4.            Pokora, I., Sieroń, A.L., Kłapcińska, B., Kempa, K., Pokora, Z., 2004: Heat stress response (HSR) to exogenous heat load in men. 13th International Symposium: Molecular and Physiological Aspects of Regulatory Processes of the Organism, Cracow, June 3-4, 2004: 368-369.

    5.            E. Szilman, P. Szilman, K. Solarz, P. Brewczyński and A. L. Sieroń (2004) Idetification of new antigen in Tyrophagus putrescentiae producing IgE response in patients positive for mite allergen skin tests. Eur. J. Biochem. 271, Suppl.:1. 29th Meeting of the Federation of the European Biochemical Societies, Warsaw, 26 June - 1 July 2004.

    6.            E. Szilman, P. Szilman, K. Solarz, P. Brewczyński, A. L. Sieroń (2004) Sensitization to the storage mite Tyrophagus putrescentiae in urban population of Upper Silesia. (XX Zjazd Polskiego Towarzystwa Parazytologicznego), Warszawa 2-4 wrzesień 2004

    7.            A. Auguściak, M. Lesiak, M. Dubrawska, K. Urbanek, M. Kajor, D. Gołka, A. Zborek, G.C. Kopen, A.L. Sieroń (2004) Variable expression of BMP-1 variants in human tumors. (Polski Kongres Genetyczny), Gdańsk 6-9 wrzesień 2004.

    8.            A. Sieroń, P.Stańczak:ASD - Lessons on genetic background from transgenic mice with inactive gene encoding metalloprotease, tolloid-like 1 (Tll1). Man 5483, Medical Science Monitor 2006

    9.            M. Lesiak,  A. Auguściak, A.K. Szydło, A.L. Sieroń (2006) Inhibition of enzymatic activity of procollagen C-endopeptidase inhibits angiogenesis. (FECTS XXth & ISMB meeting), Oulu, Finland, 1-5 lipiec 2006.

    10.       M. Lesiak, A. Augusciak-Duma, A. Szydlo, K. Urbanek, M. Kajor, A. L. Sieron (2006) Procollagen C-endopeptidase suplice wariant mTLD activity is essential for angiogenesis and with BMP-1/His wariant could  be putative marker sof certain  types of  tumors. (Metzincin Metalloproteases in Health and Disease), Ascona, Szwajcaria, 24-29 IX 2006

    11.       Grzybowska-Chlebowczyk U., Woś H., Sieroń A.L., Więcek S., Auguściak-Duma A., Koryciak-Komarska H., Kajor M. Częstość występowania mutacji genu NOD2/CARD15 oraz ocena korelacji z obrazem klinicznym u dzieci z nieswoistymi zapaleniami jelit. Pediatr.Współcz.Gastroenterol.Hepatol.Żyw.Dziecka 2008 T.10 supl.1: 5 Ogólnopolskie Sympozjum Polskiego Towarzystwa Gastroenterologii, Hepatologii i Żywienia Dzieci, Poznań 16-18.05.2008, [streszcz]. p.33[23].

    12.       Klama-Baryła A., Sieroń A.L., Wajand M., Kawecki M., Nowak M., Glik J. Optymalizacja izolacji hodowli komórek naskórka (keratynocytów) pobranych od pacjenta. Leczenie Ran 2008 T.5 z.2 s.66. Uwagi: 14 Sympozjum "Oparzenia 2008", Poznań, 5-7.06.2008 r., streszcz.

    13.       Kawecki M., Glik J., Klama-Baryła A., Wróblewski P., Nowak M., Sieroń A.L. Tytuł oryginału: Miejsce substytutu skóry Biobrane (R) w przygotowaniu ciężko oparzonego do przeszczepów autologicznych keratynocytów hodowlanych in vitro. Leczenie Ran 2008 T.5 z.2 s.66. Uwagi: 14 Sympozjum "Oparzenia 2008", Poznań, 5-7.06.2008 r., streszcz.

    14.       Sieroń A.L. Defekty genów kodujących składniki macierzy pozakomórkowej i czynniki kontrolujące jej jakość; implikacje diagnostyczne i terapeutyczne. Źródło: W: 18 Sympozjum Sekcji Ortopedii Dziecięcej Polskiego Towarzystwa Ortopedycznego i Traumatologicznego. Białystok, 29-31.05.2008. Program i streszcz. prac. [3]

    15.       Szydło A.K., Tarnowski M., Kurcok P., Kawalec M., Sobota M., Kruczek A., Sieroń A.L. Growing mesenchymal cells in artificial scaffolds enriched with procollagen I. W: 21st Meeting of the Federation of the European Connective Tissue Societies. Marseille, 9-13.07.2008. Programme and abstr. p.155[H7P]

    16.       Auguściak-Duma A., Lesiak M., Urbanek K., Kajor M., Zborek A., Piwowarczyk M., Sikora J., Sieroń A.L. Identification of procollagen C-endopeptidase mRNA splicing variants in leiomyomas. W: 21st Meeting of the Federation of the European Connective Tissue Societies. Marseille, 9-13.07.2008. Programme and abstr. p.165[J11P]

    17.       Sieroń A.L., Stańczak P., Witecka J., Szydło A., Auguściak-Duma A., Gutmajster E., Lisik M., Tarnowski M. Mutations in mammalian tolloid-like 1 gene detected in adult patients with ASD. W: 21st Meeting of the Federation of the European Connective Tissue Societies. Marseille, 9-13.07.2008. Programme and abstr. p.139[G7P]

    18.       Lesiak M., Auguściak-Duma A., Szydło A., Urbanek K., Sieroń A.L. Specific inhibition of procollagen C-endopeptidase activity by synthetic peptide with conservative sequence found in chordin. W: 21st Meeting of the Federation of the European Connective Tissue Societies. Marseille, 9-13.07.2008. Programme and abstr. p.164[J10P]

    19.       Sieroń A.L., Tarnowski M., Szydło A. Technology for fixing collagen type 1 genes in stem cells by genetic engineering and homologous recombination. W: 21st Meeting of the Federation of the European Connective Tissue Societies. Marseille, 9-13.07.2008. Programme and abstr. p.111[E7P]

    20.       Auguściak-Duma A., Witecka J., Kruczek A., Szydło A., Lesiak M., Krzak M., Pietrzyk J.J., Mannikko M., Pihlajaniemi T., Sieroń A.L. Mutations including two newly identified in patients with osteogenesis imperfecta (OI) localize in sequences of COLIA1 encoding regions that stabilize the collagen type 1 triplehelix. W: 21st Meeting of the Federation of the European Connective Tissue Societies. Marseille, 9-13.07.2008. Programme and abstr. p.113[E11P]

    21.       Grzybowska-Chlebowczyk U., Woś H., Sieroń A.L., Kasznia-Kocot J., Kajor M., Auguściak-Duma A., Koryciak-Komarska H., Więcek S., Paleń P. Serologic investigations in children with inflammatory bowel disease and food allergy. W: Falk Symposium No 164: Intestinal Disorders, Budapest [Hungary] 2-3.05.2008, abstr. p.41.

    22.       Sieroń AL, Mierzewska-Cisowska A, Kawecki M, Nowak M, Klama-Baryła A, Wajand M, Koryciak-Komarska H, Szydło A, Pruszczyńska K. Biological materials for skin wound healing: From skin cell suspensions to engineered skin-like equivalents. BIT's 1st Annual World Congress of Regenerative Medicine & Stem Cell 2008, Foshan, China, 2008 Dec. 2-4. Abstr. s

Patenty:
  1. Prockop D.J., Ala-Kokko L., Fertala A., Sieron A., Kivirikko K.I., Geddis A. (1995) Synthesis of human procollagens and collagens in recombinant DNA systems. United States Patent, Patent Number 5,405,757. Apr. 11, 1995.
  2. Prockop D.J., Hojima Y., Li S.-W., Sieron A. (2000) Recombinant C-proteinase and processes, methods and uses thereof. United States Patent, Patent Number 6,020,193. Feb. 1, 2000.